Strategies overcome Multi-drug resistance in HIV: GAPDH as a restriction factor to suppress the packaging of the primer tRNA for reverse transcription

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K18922
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Environmental and hygienic pharmacy
• Research Institution: Kumamoto University
• Principal Investigator: KISHIMOTO Naoki 熊本大学, 大学院生命科学研究部(薬), 助教 (80756148)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: HIV / 宿主因子 / 逆転写反応 / tRNA

Outline of Final Research Achievements

GAPDH, a host protein, suppresses the packaging of tRNALys3, which is required for effective viral reverse transcription as a primer. Here, I investigated the mechanism of tRNALys3-packaging suppression by GAPDH. As results, GAPDH interacts with both matrix region and capsid region of viral precursor protein. Interestingly, GAPDH oligomerization prevents tRNALys3 packaging into virions. These findings indicate that utilization of the role of GAPDH in tRNALys3 packaging is effective for preventing initiation of HIV reverse transcription.

Free Research Field

ウイルス学