2017 Fiscal Year Final Research Report
Study on epidemiology and pathology of Helicobacter cinaedi
| Project/Area Number |
16K18923
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental and hygienic pharmacy
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| Research Institution | Aichi Gakuin University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | ヘリコバクター・シネディ / 疫学 |
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| Outline of Final Research Achievements |
The CRISPR-Cas system provides adaptive immunity against foreign genetic elements. I investigated the CRISPR-Cas system to assess the potential of the CRISPR-based microevolution of Helicobacter cinaedi strains. Analysis of the organization and sequence similarity of the spacers of the H. cinaedi strains showed that CRISPR arrays could be divided into 7 different genotypes. Each genotype had a different ancestral spacer, and spacer acquisition/deletion events occurred while isolates were spreading. Spacer polymorphisms of conserved arrays across the strains were instrumental for differentiating closely-related strains collected from the same hospital. CRISPR sequences constitute a valuable basis for genotyping, provide insights into the divergence and relatedness between closely-related strains.
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| Free Research Field |
細菌分類学
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