Investigation of AQP0 functions for making anti-cataract drug that targets for AQP0

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K18957
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Medical pharmacy
• Research Institution: Keio University
• Principal Investigator: Nakazawa Yosuke 慶應義塾大学, 薬学部(芝共立), 助教 (60411708)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 水晶体 / 水チャネル / 白内障 / 細胞接着

Outline of Final Research Achievements

(1) The permeation assay of AQP0 was used the stable L-cell line expressing AQP0. As the result, AQP0 can permeate ascorbic acid ex vivo, and also indicate that there is a difference between the import and export of ascorbic acid via AQP0 channel.
(2) The cell adhesion assay of AQP0 was revealed that AQP0 could bind directly to the opposing membrane through the C-loop domain. We also found that 109Pro and 110Pro were important amino acids for cell adhesion, but mutations in the C-loop of AQP0 did not affect AQP0 water permeability.

Free Research Field

医療薬学、生物学