The physiological ligand and activation mechanisms of an orphan metabotropic receptor Prrt3

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K18999
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General physiology
• Research Institution: National Institute for Physiological Sciences
• Principal Investigator: Chen I-Shan 生理学研究所, 分子細胞生理研究領域, 特任助教 (40757770)
• Research Collaborator: KUBO Yoshihiro 生理学研究所, 教授 ; TATEYAMA Michihiro 生理学研究所, 准教授 ; YAMAMOTO Tomomi 生理学研究所, 技術職員 ; FUKATA Yuko 生理学研究所, 准教授 ; UESUGI Motonari 京都大学, 教授
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: Ligand / GPCR

Outline of Final Research Achievements

Prrt3 is an orphan family C metabotropic receptor. There is no publication concerning Prrt3 and the physiological roles and functioning mechanisms of Prrt3 are totally unknown. In the present study, we aim to clarify the physiological ligand and activation mechanisms of Prrt3. We performed molecular biological, electrophysiological and [Ca2+]i imaging experiments using Xenopus oocytes and HEK293 cells, and we observed the following results: (1) Gi/o, but not Gq, is coupled with Prrt3; (2) Prrt3 is not activated by the cleavage of its N-terminal extracellular domain; (3) some muscarinic receptor agonists, but not ACh, slightly activates the Prrt3; (4) we happened to identify a novel activator of GIRK channel, ivermectin.

Free Research Field

生理学