2017 Fiscal Year Final Research Report
Analysis of the unique voltage-dependent mechanism of a Na+ channel by voltage-clamp fluorometry
| Project/Area Number |
16K19000
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General physiology
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| Research Institution | National Institute for Physiological Sciences |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | イオンチャネル / Na+チャネル / Two-pore channel / TPC / Nav / 膜電位依存性 / 光生理学 |
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| Outline of Final Research Achievements |
We analyzed the Two-pore Na+ channel 3 (XtTPC3) to reveal the different roles of two voltage sensor domains using the electrophysiological technique. We found that the domain-II contributes to the voltage-dependence more than domain-I in XtTPC3. In addition, the unique property of XtTPC3, in which long and high voltage stimulus gradually increases the current amplitudes of XtTPC3, was found to be dependent on a type of phosphoinositide. Furthermore, we revealed that the positively charged cluster in S4/S5 linker and helix S6 of domain-I is indispensable for the phosphoinositide dependence of XtTPC3.
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| Free Research Field |
イオンチャネルの分子生理学
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