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2016 Fiscal Year Research-status Report

Beneficial role of plasmalogens in the brain which are reduced by ageing, stress and neuroinflammation

Research Project

Project/Area Number 16K19007
Research InstitutionKyushu University

Principal Investigator

Hossain Shamim  九州大学, 医学研究院, 准教授 (40647707)

Project Period (FY) 2016-04-01 – 2018-03-31
Keywordsplasmalogens / hippocampus / neurotrophic factor / spatial learning / orphan GPR / NF-kB / c-Myc
Outline of Annual Research Achievements

In the first year, I could find out that the Plasmalogens (Pls) diet in the mice increased the hippocampal dependent memory. This memory improvement was associated with the increased expression of hippocampal BDNF expression and enhanced localization of TrkB receptors into the lipid rafts. I also found that the Pls diet reduces the amyloid beta (Aβ) protein deposition in the triple transgenic Alzheimer’s disease (AD) mice model. These results have been summarized in a paper and submitted to the journal of Nature Communication. I have further found that Pls enhance the ERK signaling through the G protein-coupled receptors (GPR1, GPR19, GPR21, GPR27 and GPR61). These data have already been published last year (Hossain MS et al., Plos One, e0150846, 2016). In addition, I have also published another paper in the Journal of Neuroscience recently (Hossain MS et al., J Neurosci. 37(15): 4074-4092, 2017.) describing our findings that the reduction of cellular Pls levels in the glial cells lead to the inflammation responses. In this study, I have explained in detail of how the GNPAT expression is reduced by inflammation, ageing and stress responses through the NF-kB and c-Myc. To the establishment of CRISPR-mediated knockout mice of the GPR19 and GPR61, I did not get any positive mice line. This could be due to the embryonic lethal effect of the knockout of these GPCRs. Further experiments are going on to demonstrate the possible interaction of Pls with the GPCRs and their biological role in the brain.

Current Status of Research Progress
Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

To the establishment of CRISPR-mediated knockout mice of the GPR19 and GPR61, I did not get any positive mice line which was confirmed by genotyping and western blotting assays using tail tissue samples. This could be due to the embryonic lethal effect of the knockout of these GPCRs.

Strategy for Future Research Activity

There will be no big changes in this project.
For the time being, I will do the best to establish the knockout mice of the orphan GPR 19 and 61.

Causes of Carryover

Because of unexpected attendance in the international meeting, which was invited as a keynote speaker, the amount of travel expense became over to the planned amount of money. Then I tried to save money for reagents. As a result, small amount of money was even left and should be used next year.

Expenditure Plan for Carryover Budget

I will use the left money from this year, because the next year is the last of this project.

  • Research Products

    (9 results)

All 2017 2016

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 4 results,  Acknowledgement Compliant: 3 results) Presentation (4 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results)

  • [Journal Article] Glucose can epigenetically alter the gene expression of neurotrophic factors in the murine brain cells.2017

    • Author(s)
      Hossain MS, Oomura Y, Katafuchi, T.
    • Journal Title

      Molecular Neurobiology

      Volume: 印刷中 Pages: 印刷中

    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Reduction of ether-type glycerophospholipids, plasmalogens, by NF-B signal leading to microglial activation.2017

    • Author(s)
      Hossain MS, Abe Y, Ali F, Youssef M, Honsho M, Fujiki Y, Katafuchi, T.
    • Journal Title

      Journal of Neuroscience

      Volume: 37 (15) Pages: 4074-4092

    • DOI

      10.1523/JNEUROSCI.3941-15.2017

    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Neuronal orphan G-protein coupled receptor proteins mediate plasmalogens-induced activation of ERK and Akt signaling.2016

    • Author(s)
      Hossain MS, Mineno K, Katafuchi T.
    • Journal Title

      PLoS ONE

      Volume: 11(3) Pages: e0150846

    • DOI

      110.1371/journal.pone.0150846

    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Roles of brain lipids in glial activation.2016

    • Author(s)
      Hossain MS, Katafuchi T.
    • Journal Title

      Advances in Neuroimmune Biology

      Volume: 6(2) Pages: 61-67

    • Peer Reviewed / Open Access
  • [Journal Article] Functional involvement of brain lipids in the neuronal survival and anti-neuroinflammation.2016

    • Author(s)
      Hossain MS, Katafuchi T
    • Journal Title

      Journal of Fatigue Science

      Volume: 11 (2) Pages: 7-17

    • Peer Reviewed
  • [Presentation] Plasmalogens synthesis in the brain is down regulated during neuroinflammation.2017

    • Author(s)
      Hossain MS, Ali F, Katafuchi T.
    • Organizer
      第94回日本生理学会大会
    • Place of Presentation
      アクトシティ浜松(浜松)
    • Year and Date
      2017-03-28 – 2017-03-30
  • [Presentation] Plasmalogens improve hippocampal dependent memory by activating BDNF-TrKB signaling in the hippocampus.2016

    • Author(s)
      Hossain MS, Katafuchi T.
    • Organizer
      The 8th International Conference on Research in Science and Technology (ICRST).
    • Place of Presentation
      Flora Grand Hotel (ドバイ United Arab Emirates (UAE)
    • Year and Date
      2016-12-20 – 2016-12-21
    • Int'l Joint Research / Invited
  • [Presentation] Reduction of Plasmalogens in the brain is associated with microglial activation, stress and aging.2016

    • Author(s)
      Hossain MS, Abe Y, Ali F, Youssef M, Honsho M, Fujiki Y, Katafuchi T.
    • Organizer
      The 1st International Plasmalogen Symposium.
    • Place of Presentation
      九州大学百年講堂(福岡)
    • Year and Date
      2016-11-07 – 2016-11-08
    • Int'l Joint Research
  • [Presentation] The special lipids, plasmalogens, improve memory by accelerating BDNF-TrkB signaling in the hippocampus .2016

    • Author(s)
      Hossain MS, Ifuku M, Abe Y, Honsho M, Kawamura J, Miyake K, Fujiki Y, Katafuchi T.
    • Organizer
      The 39th Annual Meeting of the Japan Neuroscience Society.
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2016-07-20 – 2016-07-22

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Published: 2018-01-16  

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