2017 Fiscal Year Final Research Report
Elucidation of Arf6 signaling mechanism to generate novel anticancer drugs
| Project/Area Number |
16K19014
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General pharmacology
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Research Collaborator |
Fujii Ikuo 大阪府立大学, 大学院理学系研究科, 教授
Fujiwara Daisuke 大阪府立大学, 大学院理学系研究科, 助教
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | Arf6 |
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| Outline of Final Research Achievements |
This study was carried out to generate peptides that specifically suppress the small molecule G protein ARF6 involved in tumor angiogenesis and cancer cell invasion / metastasis. First, peptides binding to ARF6 were screened by using the phage display assay. As a result of screening using a phage library expressing a peptide consisting of random 39 amino acids, 71 kinds of peptides that bind to ARF6 were identified. The ARF6 binding ability of the identified peptides were confirmed by ELISA. Furthermore, Arf6 inhibitory ability of the identified Arf6-binding peptide were evaluated by fluorescent nucleotide exchange assay. As a result of the experiments, several peptides showed Arf6 specific inhibitory ability.
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| Free Research Field |
分子生物学
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