2017 Fiscal Year Final Research Report
Analysis of the brain circuit and mechanism for itch processing
| Project/Area Number |
16K19219
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Pain science
|
|---|
| Research Institution | Kansai Medical University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2016-04-01 – 2018-03-31
|
| Keywords | 痒み / NMDA受容体 / GluN2B / 脊髄 |
|---|
| Outline of Final Research Achievements |
Although itch and pain are intimately related and may share similar peripheral and central mechanisms, there is substantial evidence that itch and pain are transmitted via their own distinct pathways. By using mice with a knock-in mutation of the Tyr1472 site to phenylalanine of GluN2B (Y1472F-KI), we found that the phosphorylation of GluN2B subunit is important for itch transmission for various stimuli including histamine and chloroquine, although serotonin itch was transmitted by distinct pathways. To distinguish neuronal pathways in the spinal cord for each pain and itch stimulus, we conducted experiments using Tet-on/off systems or double staining with immunohistochemistry and In-situ hybridization. We also searched for brain regions concerning itch processing. We made chronic itch mouse model and analyzed c-fos expression globally in the full brain.
|
| Free Research Field |
痒み
|
