Mitochondrial DNA typing in mixtures using next generation sequencing system

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K19301
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Legal medicine
• Research Institution: Tokai University
• Principal Investigator: OCHIAI Eriko 東海大学, 医学部, 助教 (60760270)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 混合試料 / ミトコンドリアDNA / 次世代シーケンス / DNA鑑定

Outline of Final Research Achievements

Analysis of "DNA mixtures" in which DNAs of more than one person are mixed is one of important issues in forensic field, but no technical method for separating DNA types of individuals has been established yet. Therefore, mitochondrial (mt) DNA analysis using the next generation sequencer (NGS) was performed, and attempts were made to separate multiple DNA types by comparing differences in base sequence of DNA chain (read) for each molecule. When NGS analysis was performed with the amplification product of about 350 bp, the entire amplification region of both HV1 and HV2 could be detected with one read. A plurality of nucleotide polymorphisms was confirmed on the obtained read, and it was possible to distinguish different mtDNA type patterns from the combination and to be judged as a DNA type of two persons.

Free Research Field

法医学