Changes with the time of the amount of physiologically active substance in the tissue: potential application to forensic diagnosis

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K19303
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Legal medicine
• Research Institution: Osaka Medical College
• Principal Investigator: Saito Takashi 大阪医科大学, 医学部, 助教 (40764981)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 法医診断 / 死後変化 / 経時変化 / 脂肪幹細胞 / 表面マーカー

Outline of Final Research Achievements

Although postmortem stem cells may be useful for regenerative therapy and forensic diagnostics, their characteristic remain to be better understood. Adipose-derived mesenchymal stem cells (ASCs) have the capacity to differentiate through several cell lineages and are able to survive in an ischemic environment for a prolonged time. This study aimed to confirm whether human postmortem ASCs can be collected and culture-expanded from cadavers. Axilla subcutaneous adipose tissues were harvested during forensic autopsy and enzymatically digested to obtain a heterogeneous cell mixture, including the ASCs population. The mixture was seeded onto collagen-coated cell culture dishes and spindle-shaped adhesive and proliferative ASCs were confirmed. And then, the cultured cells were characterized.

Free Research Field

法医学