2017 Fiscal Year Final Research Report
Spontaneous activity and regulatory mechanisms of rectal microvessels
Project/Area Number |
16K19361
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Gastroenterology
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Research Institution | Nagoya City University |
Principal Investigator |
Mitsui Retsu 名古屋市立大学, 大学院医学研究科, 講師 (90434092)
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Project Period (FY) |
2016-04-01 – 2018-03-31
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Keywords | 細動脈 / 平滑筋 / 周皮細胞 / カルシウムイメージング / 自発活動 / Cl-チャネル / 電位依存性Ca2+チャネル / ギャップ結合 |
Outline of Final Research Achievements |
The mural cells (vascular smooth muscle cells and pericytes) of rectal arterioles periodically exhibited synchronous, spontaneous rise in intracellular Ca2+ concentration. These spontaneous Ca2+ transients depended on Ca2+ release from endoplasmic reticulum Ca2+ store and subsequent Ca2+-activated Cl- channel opening-mediated Cl- efflux which are expected to cause membrane depolarisation. The depolarisation appeared to be large enough to electrically couple the mural cells connected via gap junctions. Subsequent Ca2+ influxes through T-type (TVDCCs) and L-type (LVDCCs) voltage-dependent Ca2+ channels underlay ‘synchronous’ spontaneous Ca2+ transients among the mural cells. The openings of TVDCCs and LVDCCs increased Ca2+ transient frequency and duration, respectively. The synchronous spontaneous Ca2+ transients in the mural cells may underlie the spontaneous constrictions of arterioles to prevent the rectum from ischemic damage during the prolonged wall distension by faecal pellets.
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Free Research Field |
生理学・形態学
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