Construction of the new and efficient culture method of human iPS cell-derived hepatic progenitor-like cells

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K19370
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: Tokai University
• Principal Investigator: TSURUYA Kota 東海大学, 医学部, 助教 (00725377)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: ヒトiPS細胞 / 肝前駆細胞 / フィーダーフリー

Outline of Final Research Achievements

Among the analyses of cell surface receptors, we found that proliferative ability of human iPS cell-derived hepatic progenitor-like cells (HPCs) was significantly induced by addition of both Epidermal Growth Factor(EGF) and Neuregulin1(NRG1). Moreover, we revealed that HPCs maintained high proliferative ability on the human laminin-511 fragments as substitute for culturing on mouse embryonic fibroblasts (MEFs) as feeder cells. Based on these results, we established stably and long-term expansion culture system of human iPS cell-derived HPCs.

Free Research Field

消化器内科学