Trachea regeneration using bio trachea scaffold and iPS cells

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K19446
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Respiratory organ internal medicine
• Research Institution: Niigata University
• Principal Investigator: ZHOU Qiliang 新潟大学, 医歯学系, 助教 (10770232)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: trachea engineering / trachea scaffold / iPS cell / Centrifugation Method

Outline of Final Research Achievements

We successfully established a centrifugation method that can transplant cells onto the luminal surface of the decellularized rat trachea scaffold circumferentially. Mouse iPS cells were differentiated into definitive endoderm cells and transplanted onto the luminal surface of the decellularized tracheal matrix scaffold using this centrifugation method. F344/NJc1-rnu/rnu rats transplanted with rat trachea regenerated using mouse iPS cells survived for up to 5 weeks. Histological analysis indicated the cause of death was airway stenosis due to colonic cellular proliferation of undifferentiated iPS cells. Rats transplanted with no-cell scaffold survived for 1 month, although airway stenosis was also observed due to deficiency of tracheal cartilage. Re-epithelialization with numerous ciliated epithelial cells was observed in one of the rats transplanted with trachea bioengineered using iPS cells.

Free Research Field

tissue engineering