Role of the ubiquitin-proteasome system in acid excretion of the renal collecting ducts.

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K19493
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Kidney internal medicine
• Research Institution: Kumamoto University
• Principal Investigator: Izumi Yuichiro 熊本大学, 大学院生命科学研究部(医), 助教 (20736243)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 代謝性アシドーシス / 慢性腎臓病 / ユビキチン‐プロテアソーム系

Outline of Final Research Achievements

Nonvolatile acid is generated from diet and cell metabolism, and then excreted to urine through the intercalated cells in renal collecting ducts by aldosterone. We investigated the role of aldosterone in the regulation of Rhesus Blood Group, C Glycoprotein（Rhcg）protein. In vivo study, we found that aldosterone induced the accumulation of Rhcg. Consistent with that, aldosterone directly induced the expression of Rhcg protein in membrane fraction of the intercalated cell line. We further investigated the role of the ubiquitin-proteasome system (UPS), which is activated by metabolic acidosis, in the regulation of Rhcg protein. We found that low pH induced ubiquitination of proteins in the intercalated cell line. Using immunoprecipitation method, we found Rhcg was ubiquitinated. The results suggest that aldosterone and UPS regulate the expression of Rhcg protein through interaction of the two.

Free Research Field

腎臓生理学、腎臓病学