Construction of new in vitro blood-brain barrier (BBB) and blood-nerve barrier (BNB) model that incorporates multi-culturing system of BBB or BNB components.

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K19513
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurology
• Research Institution: Yamaguchi University
• Principal Investigator: TAKESHITA Yukio 山口大学, 医学部附属病院, 診療助教(4日/週) (70749829)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: Blood-Brain Barrier / Blood-Nerve Barrier / laminin / Neuromyelitis optica / transmigration

Outline of Final Research Achievements

We construct new Blood-Brain Barrier (BBB) and Blood-Nerve Barrier (BNB) model incorporating multi-cultured system of BBB or BNB specific laminin isoform and conditionally immortalized human BBB or BNB cell lines. Our models are the first in vitro BBB model to evaluate the crosstalk between BBB specific laminin and other BBB components. Next, we successfully generated the first multi-cultured BBB model that allows the transmigration of leukocytes under shear forces.
Using these moldels, we evaluated how Neuromyelitis optica (NMO)-IgG induced the leukocyte transmigration. NMO-IgG induces IL-6 in astrocytes via AQP4 and IL-6 signaling to ECs increases chemokines and drives leukocyte migration.

Free Research Field

神経免疫学