Study on endocrinological mechanism underlying body weight loss and improvement of glucose metabolism after bariatric surgery model rat

2019 Fiscal Year Final Research Report

• Project/Area Number: 16K19553
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Endocrinology
• Research Institution: University of Miyazaki (2018-2019); Kyoto University (2016-2017)
• Principal Investigator: MARUYAMA KEISUKE 宮崎大学, 農学部, 准教授 (20612386)
• Project Period (FY): 2016-04-01 – 2020-03-31
• Keywords: 肥満減量手術 / 肥満減量手術モデルラット / インスリン分泌 / 膵β細胞 / ペプチドホルモン

Outline of Final Research Achievements

The aim of this study was to clarify the endocrinological mechanism underlying body weight loss and improvement of glucose metabolism in the bariatric surgery model rat. We established the bariatric surgery model rat (RYGB rat) using Wistar fatty rat. Body weight of the RYGB group was reduced after surgery, and maintained approximately 90% of that of the Sham group. To evaluate the insulin secretory capacity in our RYGB model rats, we performed an oral glucose tolerance test (OGTT). Blood insulin level during the OGTT was markedly higher the RYGB group compared with Sham group. In addition, our examinations suggest that GLP-1 is partially involved in the enhancement of GSIS in RYGB rat. Therefore, we attempted to identify bioactive peptides involved in the enhancement of GSIS in this model rat using RNA-seq analysis and peptide purification with Ca2+ assay system.

Free Research Field

内分泌学

Academic Significance and Societal Importance of the Research Achievements

肥満減量手術に関する基礎研究により、体重減少、糖代謝改善のメカニズムを解明していくことは、急速に普及している肥満外科治療の有効性、安全性を検証し、さらなる発展を目指すうえで、決して欠かしてはならないものである。また、肥満減量手術の体重減少、糖代謝改善効果に関連した内因性因子の探索は、創薬へと繋がる可能性を秘めている。