Characterizing the large chromosomal deletion in 7q- myelodysplastic syndrome using human iPS cells and the genome editing system

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K19575
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Hematology
• Research Institution: Hiroshima University
• Principal Investigator: Nagamachi Akiko 広島大学, 原爆放射線医科学研究所, 助教 (20585153)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 骨髄異形成症候群

Outline of Final Research Achievements

Monosomy 7 and interstitial deletion of 7q is a well-recognized nonrandom chromosomal abnormality frequently found among patients with myelodysplastic syndromes and myeloid leukemias. To understand the effects of lacking large deletion on chromosome 7 in hematopoietic cell differentiation, I constructed in vitro model systems using human induced pluripotent stem (iPS) cells and the genome editing system. I suceeded in generating iPS cells carrying 7q that lacks various large regions using the CRISPR/Cas9 genome editing system, and plan to induce these cells to differentiate into multiple hematopoietic cells via the method of the formation of embryoid bodies to examine the hematopoietic function.

Free Research Field

血液内科学