2017 Fiscal Year Final Research Report
An analysis of HLA-LOH in relapsed tumor by using NGS after allogeneic stem cell transplantation
Project/Area Number |
16K19588
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Hematology
|
Research Institution | Tokai University |
Principal Investigator |
|
Research Collaborator |
ONIZUKA Makoto 東海大学, 医学部, 准教授 (80366012)
SHINA Takashi 東海大学, 医学部, 教授 (00317744)
KIKKAWA Eri 東海大学, 医学部, 特定研究員
SUZUKI shingo 東海大学, 医学部, 特定研究員
|
Project Period (FY) |
2016-04-01 – 2018-03-31
|
Keywords | HLA-LOH / 次世代シークエンサー / 同種移植後再発 / HLA不一致移植 |
Outline of Final Research Achievements |
The loss of heterozygosity in HLA lesion has been reported in relapsed leukemic cell after allogeneic stem cell transplantation (Allo-HSCT). Because the patients who had relapsed their disease after Allo-HSCT were apparently worth prognosis, we investigated whether new method used SS-SBT method could detect the LOH or not and compared the conventional micro-satellite sequencing (MS) method. The detection rates were 33% in MS method, however, 100% in SS-SBT method. It seems that next generation sequencing method has high power of detection for HLA-LOH.
|
Free Research Field |
血液内科学
|