Development of a Simultaneous fluorescence microscope and PET Imaging System for Awake Mice

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K19875
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Radiation science
• Research Institution: National Institutes for Quantum and Radiological Science and Technology
• Principal Investigator: Takuwa Hiroyuki 国立研究開発法人量子科学技術研究開発機構, 放射線医学総合研究所 脳機能イメージング研究部, 研究員(任常) (40508347)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: PET / 蛍光顕微鏡 / 生体脳イメージング / マルチモーダルイメージング / 画像補正

Outline of Final Research Achievements

In this study, we developed a system for simultaneous optical and PET imaging of awake mice. The key element is an open PET scanner (Open PET), which allows it to be combined with another device. A custom made florescence microscope was placed inside the open space of the open PET. The apparatus successfully simultaneous measured cerebral blood flow (CBF) and radioactivity concentration with 11C-raclopride PET.
In order to perform simultaneous FDG-PET measurement and flavoprotein autofluorescence imaging (FAI), we developed a method to correct for the effect of CBF on FAI. FAI is generally weak during changes of blood flow because hemoglobin absorbs fluorescent light. However, our correction method ignores the effects of CBF on FAI during neural activation.

Free Research Field

神経生理学、神経行動学、循環生理学