To develop a novel preservation solution for rat liver graft

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K19908
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General surgery
• Research Institution: National Center for Child Health and Development
• Principal Investigator: Shigeta Takanobu 国立研究開発法人国立成育医療研究センター, 臓器・運動器病態外科部, 医師 (60560331)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 臓器移植 / 保存液

Outline of Final Research Achievements

In first year, we established the rat orthotopic liver transplantation model. The AST, ALT and LDH after 3 hours of reperfusion in the 6 hours or 12 hours preservation, there is no liver injury suppression effect was observed in the SS-II solution group as compared with the HTK and UW solution group. In the second year, to improve liver preservation effect, we added the histidine, PEG, MgCl2 and glutamate to the SS-II solution. As a result, survival up to 3 days was obtained in the modified SS-II solution group after 24 hours preservation. However, the survival up to 14 days was obtained in the UW solution group under the same conditions. From the above results, it was impossible to confirm the effectiveness of liver preservation using SS-II preservation solution in the rat liver transplantation model. We conclude that it seems necessary to improve the fundamental component of SS-II solution for liver organ preservation.

Free Research Field

臓器移植