Establishment of culture and drug sensitivity test system of circulating tumor cell by biomaterial coating

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K19925
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Digestive surgery
• Research Institution: Osaka University
• Principal Investigator: MIYAKE Yuichiro 大阪大学, 医学系研究科, 招へい研究員 (80724260)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 末梢循環癌細胞 / シングルセル / 遺伝子変異

Outline of Final Research Achievements

In this study, we have challenged the development of single cell gene analysis of circulating tumor cell by using dielectrophoresis. As a result of spike test using colon cancer cell lines, we could collect approximately 75% cancer cells that were mixed in the blood sample. Gene mutations were also detected at the single cell level in both CK-positive and CK-negative CTCs of the patients with colorectal cancer. These findings indicate that detection system using the EpCAM antibody has limitation to collect up all CTCs.

Free Research Field

消化器外科