2017 Fiscal Year Final Research Report
Comprehensive analysis of DNA hypo methylation in Barrett's esophageal carcinoma
Project/Area Number |
16K19944
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Digestive surgery
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Research Institution | Kumamoto University |
Principal Investigator |
HARADA Kazuto 熊本大学, 医学部附属病院, 非常勤診療医師 (70608869)
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Project Period (FY) |
2016-04-01 – 2018-03-31
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Keywords | 食道腺癌 / Barrett食道 / LINE-1 / DNAメチル化 |
Outline of Final Research Achievements |
The level of long interspersed nucleotide element-1 (LINE-1) methylation has become regarded as a surrogate marker of global DNA methylation. LINE-1 methylation level was evaluated by using Bisulfite-Pyrosequencing method from frozen biopsy sample in esophageal adenocarcinoma, barrett’s esophagus and normal esophageal mucosa. We found that LINE-1 methylation level in esophageal adenocarcinoma was significantly lower than in Barrett;s esophagus and normal mucosa. Lower LINE-1 methylation group showed significantly poor prognosis compared with higher LINE-1 methylation group.
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Free Research Field |
消化器癌
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