2017 Fiscal Year Final Research Report
Mechanism of intimal hyperplasia development at valve site of human vein graft
| Project/Area Number |
16K19962
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cardiovascular surgery
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| Research Institution | Asahikawa Medical College |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | 内膜肥厚 / 静脈グラフト / 下肢動脈疾患 / 弁部 / グラフト狭窄 |
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| Outline of Final Research Achievements |
Venous valves are essential, but are prone to injury, thrombosis and fibrosis. We compared the behavior and gene expression of smooth muscle cells (SMCs) in the valve sinus vs non-valve sites to elucidate biological differences associated with vein valves. Tissue explants of fresh human saphenous veins were prepared, and the migration ofSMCs from explants of valve sinus vs non-valve sinus areas was measured. Valve SMCs demonstrated greater proliferation in tissue explants compared to non-valve SMCs. Valve SMCs also proliferated faster than non-valve SMCs in response to PDGF-BB.PDGF-FGF co-activation system caused the difference between both SMCs.This mechanism was applied to the ex-vivo, but no difference was seen cell functions between both SMCs.We finally tried gene screenign using RNA sequence, demonstrating that 37 genes were differentially expressed by valvec compared to non-valve tissue (11 veins).
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| Free Research Field |
血管外科
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