Cell based neovascularizaion combined with apelin for critical limb ischemia - Verification by aged cell -

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K19967
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cardiovascular surgery
• Research Institution: Yamaguchi University
• Principal Investigator: SAMURA Makoto 山口大学, 医学部附属病院, 診療助教(4日/週) (30773402)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: アペリン / 細胞移植治療 / 老化

Outline of Final Research Achievements

Expression of an capelin receptor, APJ, in peripheral blood mononuclear cell (PBMNC) isolated from young mice was increased by hypoxic preconditioning but not from old mice. Apelin did not up regulate expression of PDGF-BB and TGF-β in hypoxic PBMNCs isolated from old mice. Furthermore, proliferation of aged vascular smooth muscle cells treated with apelin was not accelerated in the presence of PDGF-BB unlike normal vascular smooth muscle cells.
These results suggested that apelin mediated responses were decreased in aged cells. And it was also suggested that the synergistic neovascular maturation effect via PDGF signaling in combinatorial treatment of hypoxic preconditioned PBMNCs with apelin may not be sufficiently showed in elderly patients.

Free Research Field

血管外科