Fabrication of scaffold-free cardiac construct by using bio 3D printer and analysis of function

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K19968
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cardiovascular surgery
• Research Institution: Saga University
• Principal Investigator: Arai Kenichi 佐賀大学, 医学部, 寄附講座助教 (40752960)
• Research Collaborator: Nakayama Kochi ; Kobayashi Eiji
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: バイオ3Dプリンタ / ヒトiPS細胞由来心筋細胞 / 心筋ポンプ / 組織工学 / 再生医療 / スフェロイド / スキャフォールドフリー

Outline of Final Research Achievements

Firstly, we optimized cardiac spheroids by mixing human iPS derived cardiomyocytes, endothelial cell, and fibroblasts. This result indicates that the addition of fibroblasts and endothelial cells promotes rapid cell self-organization and enhances cardiac spheroid stability with regards to shape and size. Secondly, we fabricated cardiac constructs by using the bio-3D printer. Cardiac constructs were cultured on the needle arrays for 7 days. Beating of the fused constructs was observed. After removal from the needle array, we confirmed electrical stimulation response of constructs, micro-vascular formation and the rearrangements of cardiomyocytes inside the construct.
In future, we will transplant this tubular cardiac construct to develop a new cardiac regenerative therapy.

Free Research Field

組織工学