2017 Fiscal Year Final Research Report
A novel method for estimating MGMT methylation status by using immunohistochemistry
| Project/Area Number |
16K20017
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurosurgery
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| Research Institution | Nagasaki University |
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Principal Investigator |
UMENO Tetsuya 長崎大学, 病院(医学系), 医員 (00737273)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | グリオーマ / MGMT |
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| Outline of Final Research Achievements |
The DNA repair protein MGMT causes resistance of cancer cells to alkylating agents and therefore, is a well-established predictive marker for high grade gliomas. Since MGMT is highly epigenetically regulated, MGMT promoter methylation status is taken as an indicator of MGMT silencing, predicting the outcome of glioma therapy. MGMT promoter methylation is usually determined by pyrosequencing, which is too expensive to perform as general inspection. Here, we apply a new method, named histo endonuclease-linked detection of methylation sites of DNA(HELMET), designed to detect methylation sites of DNA with a specific sequences in a tissue section.
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| Free Research Field |
脳神経外科学
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