Novel apoptosis-inducing ligand ORAIP plays a critical role in cerebral ischemia/reperfusion injury

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K20021
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurosurgery
• Research Institution: Yokohama City University
• Principal Investigator: TAKASE Hajime 横浜市立大学, 医学研究科, 共同研究員 (00549975)
• Research Collaborator: SUENAGA Jun ; KISHIMOTO Masao
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 酸化ストレス / アポトーシス / 脳虚血 / 再灌流障害

Outline of Final Research Achievements

Oxidative stress plays a critical role in the pathogenesis of ischemia/reperfusion (I/R) injury that leads to apoptosis. We previously identified a novel apoptosis inducing humoral factor in a conditioned medium of cardiac myocytes subjected to hypoxia/reoxygenation (H/R). We named this novel post-translationally modified secreted form of eIF5A as Oxidative stress-Responsive Apoptosis Inducing Protein (ORAIP). We confirmed that rat myocardial I/R injury was significantly suppressed by treatment with neutralizing anti-ORAIP monoclonal antibodies (mAbs). The purpose of this study is to investigate whether the same mechanism is involved in cerebral I/R injury. We found the expression of ORAIP in various types of neural cells in the ischemic penumbra region after cerebral I/R. Anti-ORAIP mAb-treatment also significantly decreased the cerebral infarct volume compared with vehicle-treatment group.

Free Research Field

外科系臨床医学・脳神経外科学