2017 Fiscal Year Final Research Report
Novel therapeutic approach for glioblastoma with iPS cells-derived CAR-T cells
| Project/Area Number |
16K20022
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurosurgery
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| Research Institution | Nara Medical University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
|
| Keywords | 遺伝子免疫細胞治療 / CAR / 膠芽腫 / iPS細胞 / T細胞 |
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| Outline of Final Research Achievements |
In this study, we aim to establish EGFRvIII-specific CAR (EvCAR)-expressing iPS cells and differentiate into T cells from the EvCAR-iPS cells. The EvCAR-expressing iPS cells were established by transducing EvCAR gene-carrying lentivirus into iPS cells. Hematopoietic stem cells (HSC) were induced from the EvCAR-iPS cells in the presence of the defined cytokines under the feedeless condition. CD3 positive and EvCAR positive cells were induced from the HSC in the presence of the defined cytokines on OP9Δ1 cells.
In conclusion, we can induce the iPS cell-derived EvCAR-T cells in vitro. The EvCAR-T cells can be a promising anti-tumoral cell source for glioblastoma if their anti-tumor effects for glioblastoma are able to be evaluate.
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| Free Research Field |
腫瘍免疫学、分子生物学
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