Analysis of heterotopic ociffication in skeletal muscle

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K20067
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Saitama Medical University
• Principal Investigator: Kuratani Mai 埼玉医科大学, 医学部, ポストドクター (50758109)
• Research Collaborator: KATAGIRI Takenobu 埼玉医科大学, 医学部, 教授 ; TSUKAMOTO Sho 埼玉医科大学, 医学部, 助教
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 骨格筋 / 異所性骨化 / BMP / 進行性骨化性線維異形成症 / 軟骨・骨

Outline of Final Research Achievements

Fibrodysplasia ossificans progressiva (FOP) is a rare genetic disorder characterized by progressive heterotopic ossification (HO) in soft tissues, such as skeletal muscle, especially after trauma. A gain-of-function mutation in ALK2, ALK2(R206H), is commonly found in patients with typical FOP. ALK2 is a type I receptor of BMP and transduces osteogenic signaling. In the present study, we observed that expression levels of BMP-2 and BMP-7 increased in injured muscle. In vivo implantation of BMP-2 and BMP-7-containing pellets drove HO in skeletal muscle. We analyzed their biological activity on ALK2(R206H) in vitro, BMP-7, induced BMP-specific luciferase reporter activities in C2C12 cells expressing human ALK2(R206H). In conclusion, BMP-7, which increased in injured muscle, contribute to the muscle trauma-induced HO in FOP.

Free Research Field

病態生理学