Comprehensive analysis of OA-related gene using NGS and pathophysiological mechanism revealed by 5-hmc status change

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K20075
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Okayama University
• Principal Investigator: Hasei Joe 岡山大学, 大学病院, 医員 (40636213)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: TWIST1 / 5hmC / 軟骨 / OA / MMPs

Outline of Final Research Achievements

It is suggested that TWIST1 is highly expressed in human OA cartilage in the next generation RNA sequence, and expression of TWIST1 increased 10-fold in OA cartilage tissue as compared with normal cartilage tissue. Expression of MMP1 and MMP3 was elevated by overexpressing TWIST1 in human chondrocytes. Overexpression of TWIST1 in a human immortalized chondrocyte cell line confirmed a 5hmC status increase in the MMP3 promoter. When TWIST1 was constitutively overexpressed in TC28, TET1 expression increased and TWIST1 expression was induced in TET triple KO fibroblast, the induction of MMP3 expression was suppressed in TET triple KO cells.

Free Research Field

骨軟部腫瘍　軟骨代謝