Comprehensive analysis of microRNA in serum specific exosomes for renal cell carcinoma enables to detect novel therapeutic target.

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K20134
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Urology
• Research Institution: Osaka University
• Principal Investigator: NAKATA Wataru 大阪大学, 医学系研究科, 招へい教員 (30648019)
• Research Collaborator: UEMURA Motohide 大阪大学, 医学系研究科, 寄附講座准教授 (40631015) ; NONOMURA Norio 大阪大学, 医学系研究科, 教授 (30263263)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 腎癌 / 腎細胞癌 / エクソソーム / マイクロRNA

Outline of Final Research Achievements

Reliable biomarkers for renal cell carcinoma (RCC) have yet to be found. Recently, exosome is getting more attention as an emerging biomarker for several types of cancer. Exosomes are lipid bilayer vesicles containing protein, messengerRNA and microRNA (miRNA). The aim of this study is to construct novel diagnostic model for RCC using miRNAs in serum exosomes. We performed miRNAs microarray analysis of exosomes and identified miRNAs that expressed more highly in exosomes in RCC patients compared to those in healthy volunteer. Novel diagnostic model constructed by random forest analysis had a good capability (sensitivity 97.8%, specificity 50.0%) for the diagnosis of RCC.

Free Research Field

泌尿器腫瘍