2017 Fiscal Year Final Research Report
Usefulness of disease-specific microRNA for respiratory syncytial virus induced lower respiratory tract inflammation
| Project/Area Number |
16K20266
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
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| Research Collaborator |
HIMI Tetsuo 札幌医科大学, 医学部, 名誉教授 (90181114)
YOKOTA Shin-ichi 札幌医科大学, 医学部, 教授 (10325863)
Takano Kenichi 札幌医科大学, 医学部, 准教授 (70404689)
KUROSE Makoto 札幌医科大学, 医学部, 講師 (60404696)
KAMEKURA Ryuta 札幌医科大学, 医学部, 講師 (70404697)
OHKUNI Tsuyoshi 札幌医科大学, 医学部, 助教 (40464490)
OGASAWARA Noriko 札幌医科大学, 医学部, 助教 (00438061)
YAMAMOTO Soh 札幌医科大学, 医学部, 助教 (10588479)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | RSウイルス / III型インターフェロン / クラリスロマイシン / IRF-3 / 鼻汁 / microRNA / housekeeping gene |
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| Outline of Final Research Achievements |
(1)Clarithromycin (CAM) as a treatment of RSV infection and (2)disease-specific microRNA as an index of disease evaluation were examined.CAM treatment led to a significant reduction in RSV-mediated IL-8, CCL5, IFN-βand -λ production.Furthermore,IFN-β promoter activity (activated by poly I:C and RSV infection) was significantly reduced after treatment with CAM.CAM also inhibited IRF-3 dimerization and subsequent translocation to the nucleus.
RSV infection-specific secreted miRNAs were identified. Housekeeping gene of nasal secreted miRNA was identified.
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| Free Research Field |
上気道感染症
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