2017 Fiscal Year Final Research Report
Light-induced mitochondria damage and cell death in the retina
| Project/Area Number |
16K20329
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Ophthalmology
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| Research Institution | Keio University |
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Principal Investigator |
Toda Eriko 慶應義塾大学, 医学部(信濃町), 研究員 (90722992)
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| Research Collaborator |
OZAWA Yoko
KAWASHIMA Hirohiko
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | 網膜 / ミトコンドリア / 光障害 |
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| Outline of Final Research Achievements |
Six-week old Balb/c male mice were exposed to the light after dark adaptation, and the cell death in the retina was analyzed using TUNEL assay. The TUNEL positive and apoptotic cells were increased only in the photoreceptor layer 2 days after light exposure. The TUNEL staining was positive in the nuclei which were defined by the staining with Dapi. The mice were treated with an antioxidant, which is supposed to affect mitochondria, before light exposure to analyze the effects on the apoptosis, although the significant effect was not detected.
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| Free Research Field |
眼科学
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