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2017 Fiscal Year Final Research Report

Identification of novel biding partners for oncogenes of Ewing sarcoma

Research Project

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Project/Area Number 16K20345
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Pediatric surgery
Research InstitutionYamaguchi University

Principal Investigator

KITAGAWA Takao  山口大学, 大学院医学系研究科, 助教 (20614928)

Project Period (FY) 2016-04-01 – 2018-03-31
KeywordsBioID / プロテオミクス / ユーイング肉腫
Outline of Final Research Achievements

Ewing sarcoma is a bone or soft tissue cancer, which is caused by chromosomal rearrangement. The resulting EWS-FLI1, EWS-ERG, EWS-E1AF show oncogenic activity, but its molecular mechanism is almost unclear. To identify binding partners for EWS-FLI1, I performed Two-hybrid screening for human cDNA and co-immunoprecipitation of EWS-FLI1. As a result, no binding partners were obtained in the two-hybrid screening, but RBM14 was obtained in the co-immunoprecipitation screening. Further, I tried BioID method, which is proximity biotination assay for target proteins. I obtained SFPQ, NONO, PSPC1, and FUS, which are part of paraspecle components, as a interaction partner. However, these proteins did not show any interaction for EWS-FLI1. Now, I have tried development of piggybac system of BioID method for EWS-FLI1.

Free Research Field

癌生物学

URL: 

Published: 2019-03-29  

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