2017 Fiscal Year Final Research Report
The effect of a carbon dioxide laser on wound healing for intractable skin ulcer
| Project/Area Number |
16K20369
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Plastic surgery
|
|---|
| Research Institution | Juntendo University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2016-04-01 – 2018-03-31
|
| Keywords | 炭酸ガスレーザー / 低出力照射治療 / 線維芽細胞 / 創傷治癒 / シグナル伝達分子 |
|---|
| Outline of Final Research Achievements |
Although Low-level laser therapy (LLLT) with a carbon dioxide (CO2) laser was also reported to promote wound healing, the underlying mechanisms at the cellular level have not been previously described. Herein, we investigated the effect of LLLT with a CO2 laser on fibroblast proliferation and migration.
In MTS and cell migration assays, fibroblast proliferation and migration were promoted after LLLT with a CO2 laser at 1.0 J/cm2. Western blot analysis revealed that Akt, ERK, and JNK activities were promoted in fibroblasts after LLLT with a CO2 laser at 1.0 J/cm2.Moreover, inhibition of Akt, ERK, or JNK significantly blocked fibroblast proliferation and migration.These findings suggested that LLLT with a CO2 laser would accelerate wound healing by promoting the proliferation and migration of fibroblasts. Activation of Akt, ERK, and JNK was essential for CO2 laser-induced proliferation and migration of fibroblasts.
|
| Free Research Field |
形成外科
|
