2017 Fiscal Year Final Research Report
IL-1alpha inducing activity of Streptococcus sanguinis
| Project/Area Number |
16K20432
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pathobiological dentistry/Dental radiology
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| Research Institution | Hokkaido University |
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Principal Investigator |
Saeki Ayumi 北海道大学, 歯学研究院, 助教 (70638345)
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| Research Collaborator |
SHIBATA Ken-ichiro
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | S. sanguinis / IL-1α / インフラマソーム |
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| Outline of Final Research Achievements |
S. sanguinis had the activity to induce secretion of IL-1α by a murine dendritic cell (XS-106) and a murine bone marrow-derived macrophage (BMMs) from C57BL/6 in a dose-dependent manner, but the activity toward NLRP3-, ASC- and caspase-1-deficient BMMs were significantly attenuated. The calpain-1 inhibitor MDL28170 but not the caspase 1 inhibitor z-YVAD-fmk attenuated the IL-1α-inducing activity. Cytochalasin D, an inhibitor of actin polymerization reduced the IL-1α-inducing activity. The receptor P2X/P2Y inhibitor oxidized ATP downregulated IL-1α release. Colocalization of caspase-1 with IL-1α was detected by Proximity ligation assay.
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| Free Research Field |
歯学
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