Establishment of periodontal ligament stem cells derived human induced pluripotent stem cells and effective regeneration of periodontal ligament tissues using these cells.

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K20457
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Conservative dentistry
• Research Institution: Kyushu University
• Principal Investigator: Hamano Sayuri 九州大学, 歯学研究院, 助教 (40757978)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: iPS細胞 / 歯根膜幹細胞 / 細胞外マトリックス

Outline of Final Research Achievements

In this study, we aimed to induce the differentiation of induced pluripotent stem (iPS) cells into periodontal ligament stem cells (PDLSCs) and evaluate their regenerative potential for periodontal ligament (PDL) tissues. After the culture of neural crest-like cells derived from iPS (iPS-NC) cells on the extracellular　matrix (ECM) from human PDL cells, they showed the up-regulation of the expression of PDL and mesenchymal stem cell markers. When iPS-NC cells exposed to PDL cells-derived ECM were transplanted into rat PDL defects, they promoted the formation of new PDL- and alveolar bone-like tissues. These results suggested our culture method was crucially efficient to generate PDLSCs-like cells from iPS cells and these cells had the potential to induce PDL tissue regeneration.

Free Research Field

歯科保存学