Fundamental study on bone regeneration by allogeneic adipose-derived stem cells utilizing immunotolerance of stem cells

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K20560
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: The University of Tokyo
• Principal Investigator: Takahashi Naoko 東京大学, 医学部附属病院, 病院診療医 (10569635)
• Research Collaborator: TAKATO Tshuyoshi ; HOSHI Kazuto ; KANAZAWA Sanshiro ; UTO Sakura
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 脂肪由来間葉系幹細胞 / 免疫寛容 / 次世代シーケンサー

Outline of Final Research Achievements

We examined the factors associated in immune tolerance by cytokine assay. However, ASC isolated by FACS was remained an issue, as these cells were heterogeneity cell population. Therefore, we verified whether there are cells involved in immune tolerance in the population using single cell RNA-Seq. As a result, ASC isolated by the conventionally method revealed that were consists of ten populations from genetic similarity. Furthermore, through global gene analysis, expression of a genes related in an immune tolerance were upregulated in a specific cell population X. When this cell population was co-cultured with CD3-positive T cells in LPS-supplemented medium, we confirmed differentiation of CD3 positive T cells to CD4/25/Foxp3 positive Treg was significantly increased. In addition, the signal existing between population X and CD3 positive T cells was analyzed by RNA-Seq. As a result, we identified candidate factors associated in the immune tolerance.

Free Research Field

再生医療