2017 Fiscal Year Final Research Report
Establishment and characterization of oral and maxillofacial disease-specific human iPSCs in serum-, integration- and feeder-free culture
| Project/Area Number |
16K20580
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Surgical dentistry
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | 細胞培養 / 再生医療 / 遺伝子疾患 |
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| Outline of Final Research Achievements |
In this study, we have demonstrated the generation of the disease-specific human iPSCs from oral and maxillofacial disease in completely serum-, feeder-, and integration-free culture. We have successfully generated iPSCs from PBMCs of CCD-iPSCs, Noonan syndrome, Neurofibromatosis type I, Nevoid Basal Cell Carcinoma Syndrome and Cowden syndrome patients in integration-, feeder cell-, and serum-free defined culture. In CCD- and Noonan-iPSCs, there were difference in cartilageous elements between wild type and CCD and Noonan iPSCs in chondrogenesis. This difference observed was similar to those symptoms of these patients. Using these disease-specific iPSCs would provide a powerful platform for examining the pathophysiology of relevant diseases at the cellular and molecular levels and for developing new drugs and therapeutic regimens.
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| Free Research Field |
口腔外科学
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