2017 Fiscal Year Final Research Report
The elucidation of the mechanism of various forms of collective cellular invasion in ameloblastoma
| Project/Area Number |
16K20596
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Surgical dentistry
|
|---|
| Research Institution | Kagoshima University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2016-04-01 – 2018-03-31
|
| Keywords | エナメル上皮腫 / 歯原性腫瘍 / 間質線維芽細胞 / ３次元培養 / 集団的細胞浸潤 |
|---|
| Outline of Final Research Achievements |
In order to compare the collective invasive forms of ameloblastoma cell lines AM-1 (plexiform type) and AM-3 (follicular type) of different tissue types, we performed 3D culture using Double Layered Collagen Gel Hemisphere culture (DL-CGH) method. Moreover, collective cellular invasion of ameloblastoma cells was assessed in the presence or absence of fibroblasts. Notably, without fibroblasts, AM-1 cells formed sharp, plexiform-like invasive processes, whereas AM-3 cells formed a series of blunt processes often observed during collective migration. In comparison, we could found that the presence of fibroblasts affected the invasive form of ameloblastoma cells.
|
| Free Research Field |
口腔顎顔面外科
|
