2017 Fiscal Year Final Research Report
Analysis of differentiation-inducing factors on the induction method for iPS cells using frozen sections.
| Project/Area Number |
16K20624
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Surgical dentistry
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| Research Institution | Tsurumi University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | 再生医学 / iPS細胞 / 分化誘導 |
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| Outline of Final Research Achievements |
We clearly demonstrated that iPSCs are capable of differentiating into a specific cell lineage in response to certain factors contained in frozen sections of tissues/organs. Interestingly, the differentiation efficiency of iPSCs on the frozen sections was clearly different among iPSC clones. Judging from these facts, this induction method of human iPSCs using frozen sections is considered to be useful as a simple and effective means for inducing the differentiation of iPSCs and also for evaluating the potential and/or quality of iPSCs. Our results also suggest that some factors, such as proteins and microRNA that can be inactivated/eliminated by fixation, may contribute to the induced differentiation of iPSCs on frozen sections. The cellular and molecular biological mechanisms underlying this phenomenon could be clearly elucidated in the near future.
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| Free Research Field |
再生医療
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