2017 Fiscal Year Final Research Report
Analysis for stimulation of GPR120 for osteoclast formation and orthodontic tooth movement
| Project/Area Number |
16K20637
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthodontics/Pediatric dentistry
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | GPR120 / DHA / 破骨細胞 |
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| Outline of Final Research Achievements |
PBS, LPS, LPS+DHA, LPS+DHA+AH7614, and DHA were injected subcutaneously on the calvariae of wild-type mice to confirm the LPS-induced osteoclast formation and the inhibitive effect of GPR 120 signaling pathway on LPS-induced osteoclast formation. After supracalvarial injections of reagents, mice were euthanized for preparing paraffin sections of mouse calvaria. TRAP staining was then performed to evaluate osteoclast formation in the suture mesenchyme. In the LPS injection group, many TRAP positive osteoclasts were induced. On the other hand, there was a significant reduction of osteoclast number in the LPS+DHA injection group. In addition, the inhibitive effect of DHA on osteoclast formation was suppressed by selective GPR120 antagonist AH7614, which was shown in the LPS+DHA+AH7614 injection group.
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| Free Research Field |
骨代謝
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