Compositional analysis in oral and gut microbiota

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K20692
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Social dentistry
• Research Institution: Osaka University
• Principal Investigator: Ogawa Taiji 大阪大学, 歯学研究科, 助教(常勤) (10543481)
• Research Collaborator: KAWABATA SHIGETADA 大阪大学, 大学院歯学研究科, 教授 (50273694) ; MAEDA YOSHINOBU 大阪大学, 大学院歯学研究科, 特任教授 (10144510) ; IKEBE KAZUNORI 大阪大学, 大学院歯学研究科, 准教授 (70273696) ; OGAWA MARIKO 大阪大学, 大学院歯学研究科, 特任研究員 (20754732) ; HIROSE YUJIRO 大阪大学, 大学院歯学研究科, 特任助教 (90788407)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 口腔細菌叢

Outline of Final Research Achievements

We investigated the salivary microbiota composition in samples from healthy and frail elderly individuals using 16S rRNA sequencing analysis for characterization. We found a significant difference in diversity between elderly individuals living in a nursing home (EN) and healthy control (HC) subjects, as well as in the microbiota composition at the phyla level. A supervised orthogonal partial least squared discriminant analysis (OPLS-DA) revealed a significant difference in clear classification trend between the EN and HC groups, with all observations falling within the Hotellings T2 (0.95) ellipse, with model fitness parameters of R2(cum)=0.937 and Q2(cum)=0.888, respectively. In addition, the score plots by unsupervised principal component analysis (PCA) showed a clear classification trend in both groups. Our findings suggest that general frailty is associated with oral microbiota composition and formation.

Free Research Field

高齢者歯科