2019 Fiscal Year Final Research Report
Mechanism of hypothalamic Corticotropin Releasing factor regulation by Leptin
| Project/Area Number |
16K20891
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Endocrinology
Environmental physiology(including physical medicine and nutritional physiology)
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| Research Institution | Hirosaki University (2017-2019)
Tohoku University (2016) |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2020-03-31
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| Keywords | Leptin / CRF |
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| Outline of Final Research Achievements |
In the hole cell patch-clamp recording, Venus-positive neurons in the PVH of CRF-Venus mice have glutamatergic and GABAergic inputs. In a study using CRF neuron-selective ChR2-expressing mice (ChR2-mCherry / CRF-iCreΔNeo), almost all CRF neurons expressed ChR2(mCherry). CRF neurons were depolarized and fired by blue light. Blue light irradiation of CRF neurons increased c-Fos expression in the PVH and other brain regions.
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| Free Research Field |
内分泌学
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| Academic Significance and Societal Importance of the Research Achievements |
コルチコトロピン放出因子（CRF）はストレス応答における視床下部-下垂体-副腎軸の要であると同時に，摂食に関与すると言われている．しかし詳細なメカニズムは解明されていない．またレプチンによるCRFニューロンの調節機構は明らかになっていない．CRF-VenusΔNeoマウスは, CRFニューロンが蛍光可視化されるため電気生理学的手法や形態学的検証を可能にし，CRFニューロンのはたらきを解明するための重要なツールとなる．
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