2017 Fiscal Year Final Research Report
Development of mRNA transportation mechanisms in neural stem/progenitor cells during evolution
| Project/Area Number |
16K20902
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General anatomy (including histology/embryology)
Morphology/Structure
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | 大脳皮質 / 放射状グリア細胞 / Cyclin D2 / mRNA輸送 / 進化 |
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| Outline of Final Research Achievements |
During cortical development in mammals, neural stem/progenitor cells need to adequately proliferate and differentiate. Neural progenitor cells during corticogenesis are called radial glial (RG) cells because they show highly polarized morphology with long and thin processes. We have previously shown that mRNA of Cyclin D2 encoding a cell cycle regulator is transported to the basal end-foot of RG cells by the specific mRNA transportation element. We applied a CRISPR/Cas9 genome editing system in mouse embryos and selectively removed the element. We found that the mutant mice show inhibition of Cyclin D2 mRNA transportation to the basal end-foot of RG cells. These results suggest involvement of the mammalian specific cis-regulatory sequence of Cyclin D2 mRNA in the mRNA transportation to the basal end-foot of RG cells.
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| Free Research Field |
神経発生学
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