2017 Fiscal Year Final Research Report
Establishment of molecular design guideline for hyperbrancked protein polymers and their use as scaffolds
| Project/Area Number |
16K21008
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
Bio-related chemistry
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Research Collaborator |
Patmawati Lili Jia
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | ポリマー / 蛋白質 / 複合化 / 自己集合 / セルロソーム / 検出プローブ |
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| Outline of Final Research Achievements |
Conjugating functional proteins into a large assembly often results in an enhancement of overall functionalities.Here, a SpyCatcher polymer is prepared through catalytic reaction of horseradish peroxidase (HRP) and serves as a polymeric proteinaceous scaffold for construction of protein assemblies. Taking advantage of the favorable SpyCatcher and SpyTag interaction, SpyTagged proteins can
be easily assembled onto the polymeric SpyCatcher scaffold with controllable binding ratio and site specificity. We demonstrated construction of ratio-controllable binary artificial hemicellulosomes by assembling endoxylanase and arabinofuranosidase and biosensing probe by conjugating SpyTagged Nanoluc and protein G onto SpyCatcher polymer. Through this studies, we managed to obtain knowledge of condition of protein polymer preparation and assemblies of proteins onto the protein polymers.
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| Free Research Field |
タンパク質工学
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