A new method of functional evaluation of pluripotent stem cell-derived islet

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K21116
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General surgery; Biomedical engineering/Biomaterial science and engineering
• Research Institution: Kyoto University
• Principal Investigator: Konagaya Shuhei 京都大学, ウイルス・再生医科学研究所, 特定研究員 (60770295)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 膵島 / 細胞外電位 / 多能性幹細胞 / 分化誘導

Outline of Final Research Achievements

To develop a new method of functional evaluation of pluripotent stem cell-derived islet, we have recorded the electrical activity of the cells using planar, extracellular electrodes arranged in a microelectrode array (MEA). Although isolated-mouse islet cells poorly adhered on MEA, the cells adhered well on the laminin-332-coated MEA. We succeeded in recording of the electrical activity of islet cells in response to glucose stimulation. We also recorded the activity of human iPS cell-derived islet cells. However, the electrical signal was very low and noisy. It might be caused by insufficient maturation and heterogeneity of the differentiated cells. As a solution, we newly developed a culture method for expansion and purification of pancreatic cells derived from human iPS cells.

Free Research Field

再生医療