2017 Fiscal Year Final Research Report
Search for early marker of Osteoarthritis using Exosome and establish monitoring index of exercise and nutritional effects
| Project/Area Number |
16K21192
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied health science
Laboratory animal science
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| Research Institution | Kyoto Sangyo University (2017)
The University of Tokyo (2016) |
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Principal Investigator |
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| Research Collaborator |
MIYAKI Shigeru 広島大学, 病院, 講師 (10392490)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | 変形性関節症 / microRNA / カルノシン酸 / Exosome |
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| Outline of Final Research Achievements |
In this study, we explored the effect of carnosic acid (CA) on osteoarthritis (OA) using human synovial fibroblast and human chondrocytes. The expression of heme oxygenase-1(HO-1) was upregulated in human synovial fibroblasts and chondrocytes treated with CA in a dose-dependent manner. CA had no effect on the expression of Col2a1 in chondrocytes. CA suppressed the expression of OA-related genes in human synovial fibroblast and chondrocytes.In addition, the expression of the cartilage-degrading enzyme was suppressed. We found that CA activates miR-140 which maintains cartilage homeostasis and activates HO-1 by suppressing the expression of Bach1 gene as its molecular mechanism. These findings suggest that CA is an effective HO-1 inducer and have a potential to be a supplement for OA prevention.
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| Free Research Field |
分子生物学 運動器疾患
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