High functional gene activated matrix with nanobioglass and low adhesive scaffold collagen

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K21235
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry; Dental engineering/Regenerative dentistry
• Research Institution: Nagasaki University
• Principal Investigator: MIURA Kei-ichiro 長崎大学, 医歯薬学総合研究科(歯学系), 助教 (10634446)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: Gene Activated Matrix / Bone Regeneration / LASCol / バイオマテリアル

Outline of Final Research Achievements

The purpose of this study is to develop new biomaterials that hold low dose nonviral vectors. We aimed for clinical application by improving conventional GAM with the enhancement of the cellular phagocytosis of DNA by nanobioglass, the improvement of gene transfer efficiency of plasmid DNA, and both induction of cell spheroid formation and enhancement for bone marrow mesenchymal cells to promote differentiation into osteoblasts by the low adhesive scaffold collagen as a scaffold. In order to select the appropriate concentration among plasmid DNA, nanobioglass and LasCol which possess the highest osteogenic potential, transplantation experiments were conducted on the mouse calvaria. As a result, although there was no significant difference when compared with the atelocollagen group, low adhesion collagen showed good osteogenic potential.

Free Research Field

口腔顎顔面外科学