2017 Fiscal Year Final Research Report
Comparative proteomics of FFPE tissue for Exploring biomarker of well differentiated liposarcomas
| Project/Area Number |
16K21250
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Human pathology
Tumor diagnostics
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | FFPE / ALT/WDL / Proteomics |
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| Outline of Final Research Achievements |
In this study, comparable proteome analysis of FFPE tissues revealed multiple candidate marker molecules for differentiating atypical lipomatous tumor/well-differentiated liposarcoma (ALT/WDL) from lipoma. We identified 181 unique proteins for ALT/WDL. Of the identified proteins, we finally focused on CCDC180 and LRRC4 as candidate markers of ALT/WDL. We showed that CCDC180 and LRRC4 immunohistochemistry clearly stained tumor cells of ALT/WDL and dedifferentiated liposarcoma and could differentiate them from lipoma with high accuracy. We also used cell biological methods to further examine the expression of the CCDC180 and LRRC4 in liposarcoma cells.
Their expression was associated with adipocyte differentiation and contributed to malignant potentials of liposarcoma cells.
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| Free Research Field |
医学
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